Role of tight junction-associated MARVEL protein marvelD3 in migration and epithelial-mesenchymal transition of hepatocellular carcinoma.

Abstract

MarvelD3, a recently identified tight junction membrane protein, could be associated with hepatocellular carcinoma (HCC). We aimed to investigate the role of marvelD3 in Epithelial-Mesenchymal Transition (EMT) and migration of HCC and explore the underlying molecular mechanisms. First, we assessed marvlD3 expression in HCC and normal liver tissues and found loss of marvelD3 expression was significantly correlated with the occurrence and TNM stage of HCC. Second, we detected that marvelD3 was downregulated in HCC cells with transforming growth factor β1 and snail/slug-induced EMT. Finally, we analyzed expression of marvelD3 protein was significantly associated with EMT and the NF-κB signaling pathway. Our study demonstrated that MarvelD3 inhibited EMT and migration of HCC cells along with inhibiting NF-κB signaling pathway.Abbreviations:HCC, Hepatocellular carcinoma; TJ, Tight junction; MARVEL, MAL and related proteins for vesicle trafficking and membrane link; EMT, Epithelial-mesenchymal transition; NF-κB, Nuclear factor kappa B; TAMPs, Tight junction-associated marvel proteins; TGF-β1, Transforming growth factor-β1; MMP9, matrix metallopeptidase 9; RT-PCR, Real-time PCR; IHC, Immunohistochemistry; IF, Immunofluorescence.

• Authors: Yanmeng Li, Teng Li, Donghu Zhou, Jia Wei, Zhenkun Li, Xiaojin Li, Siyu Jia, Qin Ouyang, Saiping Qi, Zhibin Chen, Bei Zhang, Jing Yu, Jidong Jia, Anjian Xu, Jian Huang
• Journal: Cell adhesion & migration (Cell Adh Migr) Vol. 15 Issue 1 Pg. 249-260 (12 2021) ISSN: 1933-6926 [Electronic] United States
• PMID: 34338154 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tight Junction Proteins

Topics

• Carcinoma, Hepatocellular (genetics)
• Cell Line, Tumor
• Epithelial-Mesenchymal Transition
• Humans
• Liver Neoplasms (genetics)
• Tight Junction Proteins (genetics)
• Tight Junctions