Abstract |
The genomic RNAs of brome mosaic virus (BMV) exhibit various tRNA-like properties, including specific tyrosylation by tyrosyl- tRNA synthetases and adenylation of the 3'-CCOH derivative by tRNA nucleotidyl transferases. We have studied the effect of numerous mutations in all domains of the tRNA-like structure of BMV RNA on tyrosylation and adenylation in vitro. Surprisingly few mutations resulted in more than 50% decrease in tyrosylation rates with either wheat germ or yeast synthetases; those mutations were at the 3' terminus, the pseudoknot, and the bases of arms B and E. The results suggest an interaction of synthetase with arm A as the analog of the aminoacyl acceptor stem of tRNAs, and arm B as the analog of the anticodon arm of tRNAs, although there is no apparent interaction with the terminal loop of arm B analogous to the interaction with the anticodon in tRNAs. Mutations at several loci resulted in large losses of adenylation activity catalyzed by wheat germ and Escherichia coli nucleotidyl transferases; those loci were the pseudoknot, the bases of arms B, C and D, and at the junctions of these arms with arm A. These studies have identified mutants specifically defective in one of the tRNA-like activities, which are appropriate for investigating the role of these activities during infection in vivo.
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Authors | T W Dreher, T C Hall |
Journal | Journal of molecular biology
(J Mol Biol)
Vol. 201
Issue 1
Pg. 41-55
(May 05 1988)
ISSN: 0022-2836 [Print] Netherlands |
PMID | 3418699
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- RNA, Viral
- Tyrosine
- RNA, Transfer
- RNA Nucleotidyltransferases
- tRNA nucleotidyltransferase
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Topics |
- Autoradiography
- Base Sequence
- DNA Mutational Analysis
- Molecular Sequence Data
- Mosaic Viruses
(genetics)
- Mutation
- Nucleic Acid Conformation
- Promoter Regions, Genetic
- RNA Nucleotidyltransferases
(metabolism)
- RNA, Transfer
(genetics, metabolism)
- RNA, Viral
(genetics, metabolism)
- Sequence Homology, Nucleic Acid
- Transcription, Genetic
- Tyrosine
(metabolism)
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