HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mutational analysis of the tRNA mimicry of brome mosaic virus RNA. Sequence and structural requirements for aminoacylation and 3'-adenylation.

Abstract
The genomic RNAs of brome mosaic virus (BMV) exhibit various tRNA-like properties, including specific tyrosylation by tyrosyl-tRNA synthetases and adenylation of the 3'-CCOH derivative by tRNA nucleotidyl transferases. We have studied the effect of numerous mutations in all domains of the tRNA-like structure of BMV RNA on tyrosylation and adenylation in vitro. Surprisingly few mutations resulted in more than 50% decrease in tyrosylation rates with either wheat germ or yeast synthetases; those mutations were at the 3' terminus, the pseudoknot, and the bases of arms B and E. The results suggest an interaction of synthetase with arm A as the analog of the aminoacyl acceptor stem of tRNAs, and arm B as the analog of the anticodon arm of tRNAs, although there is no apparent interaction with the terminal loop of arm B analogous to the interaction with the anticodon in tRNAs. Mutations at several loci resulted in large losses of adenylation activity catalyzed by wheat germ and Escherichia coli nucleotidyl transferases; those loci were the pseudoknot, the bases of arms B, C and D, and at the junctions of these arms with arm A. These studies have identified mutants specifically defective in one of the tRNA-like activities, which are appropriate for investigating the role of these activities during infection in vivo.
AuthorsT W Dreher, T C Hall
JournalJournal of molecular biology (J Mol Biol) Vol. 201 Issue 1 Pg. 41-55 (May 05 1988) ISSN: 0022-2836 [Print] Netherlands
PMID3418699 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • RNA, Viral
  • Tyrosine
  • RNA, Transfer
  • RNA Nucleotidyltransferases
  • tRNA nucleotidyltransferase
Topics
  • Autoradiography
  • Base Sequence
  • DNA Mutational Analysis
  • Molecular Sequence Data
  • Mosaic Viruses (genetics)
  • Mutation
  • Nucleic Acid Conformation
  • Promoter Regions, Genetic
  • RNA Nucleotidyltransferases (metabolism)
  • RNA, Transfer (genetics, metabolism)
  • RNA, Viral (genetics, metabolism)
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Tyrosine (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: