The First Mouse Model of Primary Osteosarcoma of the Breast.

Abstract

BACKGROUND/AIM:

Sarcomas of the breast are extremely rare malignant tumors and comprise only 5% of all sarcomas and fewer than 1% of breast cancers. Primary osteosarcoma of the breast is histologically indistinguishable from osteosarcoma of the bone. Effective therapies of this recalcitrant disease have not yet been developed.

MATERIALS AND METHODS:

A patient-derived xenograft (PDX) mouse model of primary osteosarcoma of the breast was established by subcutaneous implantation of the surgical specimen, along with surrounding normal tissue. Hematoxylin and eosin (H&E) staining was performed on paraffin-embedded histological sections of the original tumor resected from the patient and from implanted tumors that grew in nude mice.

RESULTS:

Tumors grew in 46 of 51 mice implanted with the original surgical specimen. The H&E-stained slides of the mouse-grown tumor and the original patient tumor matched, both showing large areas of spindle-shaped cells, characteristic of osteosarcoma.

CONCLUSION:

The first PDX mouse model of primary breast osteosarcoma was established which will enable testing of novel therapeutics as well as basic research of osteosarcoma of the breast.

• Authors: Nathaniel F Wu, Justin Wu, Jun Yamamoto, Yusuke Aoki, Chihiro Hozumi, Michael Bouvet, Robert M Hoffman
• Journal: In vivo (Athens, Greece) (In Vivo) 2021 Jul-Aug Vol. 35 Issue 4 Pg. 1979-1983 ISSN: 1791-7549 [Electronic] Greece
• PMID: 34182472 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Topics

• Animals
• Bone Neoplasms
• Disease Models, Animal
• Humans
• Mice
• Mice, Nude
• Osteosarcoma
• Xenograft Model Antitumor Assays