Abstract |
We reported previously the partial purification of detergent-solubilized specific tumor rejection antigen of a chemically induced sarcoma, Meth-A. During the course of the study, rabbit antiserum against a partially purified specific tumor rejection antigen preparation was raised and rendered specific by in vivo absorption. In this report we show that an antigenic molecule defined by in vivo-absorbed rabbit antiserum, which we tentatively refer to as tumor-specific surface antigen, was solublized by detergent Nonidet P40, and extensive attempts at purification were carried out by a sequence of procedures including gel filtration, isotachophoresis, and polyacrylamide gel electrophoresis. The most highly purified tumor-specific surface antigen retained some specific tumor rejection antigen activity, suggesting an association of the two activities. Both antigens were shown to have a molecular weight of about 60,000 and an electrophoretic mobility of alpha-globulin.
|
Authors | T Natori, L W Law, E Appella |
Journal | Cancer research
(Cancer Res)
Vol. 38
Issue 2
Pg. 359-64
(Feb 1978)
ISSN: 0008-5472 [Print] United States |
PMID | 340027
(Publication Type: Journal Article)
|
Chemical References |
- Antigens, Neoplasm
- H-2 Antigens
|
Topics |
- Animals
- Antigens, Neoplasm
(analysis, isolation & purification)
- Cell Membrane
(immunology)
- Electrophoresis, Polyacrylamide Gel
- Female
- Graft Rejection
- H-2 Antigens
(analysis, isolation & purification)
- Immunosorbent Techniques
- Mice
- Mice, Inbred BALB C
- Neoplasm Transplantation
- Radioimmunoassay
- Sarcoma, Experimental
(chemically induced, immunology)
|