Abstract |
Octopine and nopaline Ti-plasmids confer upon Agrobacterium tumefaciens C58C1 the ability to respond chemotactically to the vir-inducing phenolic wound exudate, acetosyringone. A. tumefaciens C58C1 containing Ti-plasmids with Tn5 insertions in virB, C, D or E exhibited marked chemotaxis towards acetosyringone. However, Ti-plasmids with mutations in virA or virG were unable to confer the responsive phenotype. Of the cosmid clones pVK219 (virAB) pVK221 (virBGC) pVK225 (virGCDE) and pVK257 (virABGC) mobilized to cured A. tumefaciens C58C1, only pVK257 bestowed acetosyringone chemotaxis. virA and virG are thus required for chemotaxis of A. tumefaciens towards acetosyringone. This suggests a multifunctional role for virA and virG: at low concentrations of acetosyringone they mediate chemotaxis and at higher concentrations they effect vir-induction.
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Authors | C H Shaw, A M Ashby, A Brown, C Royal, G J Loake, C H Shaw |
Journal | Molecular microbiology
(Mol Microbiol)
Vol. 2
Issue 3
Pg. 413-7
(May 1988)
ISSN: 0950-382X [Print] England |
PMID | 3398775
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acetophenones
- Bacterial Proteins
- Chemotactic Factors
- Membrane Proteins
- Methyl-Accepting Chemotaxis Proteins
- nopaline
- octopine
- acetosyringone
- Arginine
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Topics |
- Acetophenones
(physiology)
- Arginine
(analogs & derivatives, genetics)
- Bacterial Proteins
- Chemotactic Factors
(physiology)
- Chemotaxis
- Chromosome Mapping
- Cloning, Molecular
- Cosmids
- Gene Expression Regulation
- Membrane Proteins
(physiology)
- Methyl-Accepting Chemotaxis Proteins
- Mutation
- Plasmids
- Rhizobium
(genetics)
- Transfection
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