Dose escalation and expansion phase I studies with the tumour-targeting antibody-tumour necrosis factor fusion protein L19TNF plus doxorubicin in patients with advanced tumours, including sarcomas.

Abstract

BACKGROUND:

L19TNF is a recombinant fusion protein composed of a human antibody fragment and human tumour necrosis factor. L19TNF targets the EDB domain of oncofetal fibronectin highly expressed in tumour vasculature and induces tumour remission in mouse tumours. We summarise two phase I trials testing a combination of L19TNF with doxorubicin in patients with solid tumours, particularly soft tissue sarcomas (STS).

PATIENTS AND METHODS:

The first study, an open-label, dose-escalation and expansion phase I study of L19TNF plus doxorubicin, enrolled 27 patients. Three cohorts (10.4-17 μg/kg L19TNF) of patients received L19TNF intravenously at days 1, 3, and 5 and doxorubicin (75 mg/m2, then 60 mg/m2) on day 1 every 3 weeks. The expansion cohort enrolled patients with STS. The second study tried to re-escalate the doxorubicin dose to 75 mg/m2 with 13 μg/kg L19TNF. Among primary objectives was the establishment of a recommended dose (RD).

RESULTS:

The combination was safely applicable. Dose-limiting toxicity occurred either at 17 μg/kg L19TNF or at 75 mg/m2 doxorubicin. RD is 13 μg/kg L19TNF plus 60 mg/m2 doxorubicin. In 15 STS patients of the extension cohort evaluable for efficacy, antitumour activity was observed with complete remission in 1, partial remission in 1 and minor tumour shrinkage in 7 patients. The median overall survival for this heavily pretreated cohort was 14.9 months.

CONCLUSION:

L19TNF can be safely applied in combination with doxorubicin and induces encouraging tumour remissions in patients with soft tissue sarcomas.

• Authors: Christoph Schliemann, Teresa Hemmerle, Andrew F Berdel, Linus Angenendt, Andrea Kerkhoff, Jan P Hering, Walter Heindel, Wolfgang Hartmann, Eva Wardelmann, Sant P Chawla, Filippo de Braud, Georg Lenz, Dario Neri, Torsten Kessler, Wolfgang E Berdel
• Journal: European journal of cancer (Oxford, England : 1990) (Eur J Cancer) Vol. 150 Pg. 143-154 (06 2021) ISSN: 1879-0852 [Electronic] England
• PMID: 33901793 (Publication Type: Clinical Trial, Phase I, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2021 Elsevier Ltd. All rights reserved.

Chemical References

• Antibiotics, Antineoplastic
• Antineoplastic Agents, Immunological
• Doxorubicin

Topics

• Adult
• Aged
• Antibiotics, Antineoplastic (administration & dosage, adverse effects, pharmacokinetics)
• Antineoplastic Agents, Immunological (administration & dosage, adverse effects, pharmacokinetics)
• Antineoplastic Combined Chemotherapy Protocols (administration & dosage, adverse effects, pharmacokinetics)
• Doxorubicin (administration & dosage, adverse effects, pharmacokinetics)
• Female
• Humans
• Male
• Middle Aged
• Sarcoma (drug therapy, immunology, mortality, pathology)
• Soft Tissue Neoplasms (drug therapy, immunology, mortality, pathology)
• Time Factors
• Treatment Outcome