Flow cytometry analysis of the growth inhibitory effect of 4-hydroxy-tamoxifen on a human breast carcinoma cell line.

Abstract

Growth kinetics of BT-20 cells (an ER- cell line) have been studied with flow cytometry, cell counting and 3H-thymidine incorporation. The cell cycle lasts about 40 hours, with a maximum SG2M about 25h after seeding. 4-Hydroxy-tamoxifen (OH-Tamoxifen) (10(-7) M to 10(-5) M) inhibits BT-20 cell growth with maximum inhibition at 24-28h after plating. The inhibitory effect of OH-Tamoxifen is also demonstrated by a decrease in 3H-thymidine incorporation and by a reduction in cell number. These results suggest that OH-Tamoxifen is able to act upon an ER-cell line through a pathway which is different from that of estrogens.

• Authors: E Vicard, A Hijazi, E Muchada, C Chouvet, M Devonec, S Saez
• Journal: Anticancer research (Anticancer Res) 1988 May-Jun Vol. 8 Issue 3 Pg. 375-9 ISSN: 0250-7005 [Print] Greece
• PMID: 3389742 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm
• Tamoxifen
• afimoxifene
• Estradiol

Topics

• Breast Neoplasms (pathology)
• Carcinoma (pathology)
• Cell Cycle (drug effects)
• Cell Division (drug effects)
• DNA, Neoplasm (analysis)
• Depression, Chemical
• Estradiol (pharmacology)
• Flow Cytometry
• Humans
• Tamoxifen (analogs & derivatives, pharmacology)
• Tumor Cells, Cultured (drug effects)