Novel Peptide-Based Magnetic Nanoparticle for Mesenchymal Circulating Tumor Cells Detection.

Abstract

The monitoring of circulating tumor cells (CTCs) has recently served as a promising approach for assessing prognosis and evaluating cancer treatment. We have already developed a CTCs enrichment platform by EpCAM recognition peptide-functionalized magnetic nanoparticles (EP@MNPs). However, considering heterogeneous CTCs generated through epithelial-mesenchymal transition (EMT), mesenchymal CTCs would be missed with this method. Notably, N-cadherin, overexpressed on mesenchymal CTCs, can facilitate the migration of cancer cells. Hence, we screened a novel peptide targeting N-cadherin, NP, and developed a new CTCs isolation approach via NP@MNPs to complement EpCAM methods' deficiencies. NP@MNPs had a high capture efficiency (about 85%) of mesenchymal CTCs from spiked human blood. Subsequently, CTCs were captured and sequenced at the single-cell level via NP@MNPs and EP@MNPs, RNA profiles of which showed that epithelial and mesenchymal subgroups could be distinguished. Here, a novel CTCs isolation platform laid the foundation for mesenchymal CTCs isolation and subsequent molecular analysis.

• Authors: Fei Jia, Yuehua Wang, Zhiguo Fang, Jierong Dong, Fanghao Shi, Weikai Zhang, Zihua Wang, Zhiyuan Hu
• Journal: Analytical chemistry (Anal Chem) Vol. 93 Issue 14 Pg. 5670-5675 (04 13 2021) ISSN: 1520-6882 [Electronic] United States
• PMID: 33788544 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Epithelial Cell Adhesion Molecule
• Magnetite Nanoparticles
• Peptides

Topics

• Biomarkers, Tumor
• Cell Line, Tumor
• Epithelial Cell Adhesion Molecule
• Epithelial-Mesenchymal Transition
• Humans
• Magnetite Nanoparticles
• Neoplastic Cells, Circulating
• Peptides