Porphyrin-based covalent organic framework as bioplatfrom for detection of vascular endothelial growth factor 165 through fluorescence resonance energy transfer.

Abstract

A fluorescent aptasensor based on porphyrin-based covalent organic framework (p-COF) and carbon dots (CDs) was constructed for detecting vascular endothelial growth factor 165 (VEGF165) and for imaging of the breast cancer cell line Michigan cancer foundation-7 (MCF-7). CDs synthesized with strong photoluminescence at λ∼380 nm were used as donors to label the VEGF165-targeted aptamers (AptVEGF/CDs). Additionally, the p-COF nanostructure comprised rich functional groups of CN on the surface and π-stacking planar nanostructure, resulting in the CDs adsorption via weakly π-π stacking, hydrogen bond and the Van der Waals force. Thereby, the fluorescence resonance energy transfer (FRET) occurred due to the close distance between the p-COF network and CDs, leading to the quenching of the fluorescence feature of CDs and p-COF. In the presence of VEGF165, the G-quadruplex was formed via the specific binding between VEGF165 and aptamer. It impelled that the release of partial VEGF165-AptVEGF/CDs complex, affording the fluorescence recovery of the sensing system to some extent. Consequently, the proposed AptVEGF/CDs/p-COF fluorescence biosensor offered excellent analytical performances for the VEGF165 detection, displaying a detection limit of 20.9 fg mL-1 within a wide linear range of the VEGF165 concentration of 1.0 pg mL-1-100 ng mL-1. The developed fluorescence biosensor was also used to determine VEGF165-overexpressed in MCF-7 cancer cells. Thereby, the present work can greatly widen the application of COFs in the development of aptasensors and cancer diagnosis.

• Authors: Jing Cui, Lun Kan, Zhenzhen Li, Longyu Yang, Minghua Wang, Linghao He, Yafei Lou, Yulin Xue, Zhihong Zhang
• Journal: Talanta (Talanta) Vol. 228 Pg. 122060 (Jun 01 2021) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 33773722 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Aptamers, Nucleotide
• Metal-Organic Frameworks
• Porphyrins
• Vascular Endothelial Growth Factor A

Topics

• Aptamers, Nucleotide
• Biosensing Techniques
• Fluorescence Resonance Energy Transfer
• Humans
• Limit of Detection
• Metal-Organic Frameworks
• Porphyrins
• Quantum Dots
• Vascular Endothelial Growth Factor A