Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system.

Abstract

Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and Erns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and Erns were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-Erns, or their combination (CSFV-E2 + Erns). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID50 of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + Erns. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the Erns vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the Erns alone is not able to induce a protective immune response. Taken together, while the Erns could not confer protection against CSFV, E2 and E2 + Erns could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits.

• Authors: Qiang Wei, Yilin Bai, Yapeng Song, Yunchao Liu, Wei Yu, Yaning Sun, Li Wang, Ruiguang Deng, Guangxu Xing, Gaiping Zhang
• Journal: Virology journal (Virol J) Vol. 18 Issue 1 Pg. 44 (02 24 2021) ISSN: 1743-422X [Electronic] England
• PMID: 33627167 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Vaccines, Synthetic
• Viral Envelope Proteins
• Viral Structural Proteins
• Viral Vaccines
• glycoprotein E2, classical swine fever virus

Topics

• Animals
• Baculoviridae (genetics)
• Cell Line
• Classical Swine Fever Virus (chemistry, genetics, immunology)
• Female
• Immunogenicity, Vaccine
• Rabbits
• Sf9 Cells
• Swine
• Vaccines, Synthetic (administration & dosage, immunology)
• Viral Envelope Proteins (genetics, immunology)
• Viral Structural Proteins (genetics, immunology)
• Viral Vaccines (administration & dosage, genetics, immunology)