Optimized Tibial Nerve Stimulation Partially Reduces Visceral Hypersensitivity in Rats Mediated via Autonomic and Opioid Mechanisms.

Abstract

OBJECTIVES:

The aim of this study was to investigate the therapeutic effect and possible mechanisms of tibial nerve stimulation (TNS) on visceral hypersensitivity in rats.

MATERIALS AND METHODS:

1) The effects of TNS with five sets of parameters on visceral sensitivity in normal rats were evaluated by the assessment of abdominal electromyogram (EMG) and abdominal withdrawal reflex (AWR). 2) The effects and mechanisms of TNS with a special set of parameters (14 Hz, 330 μsec, and 40% motor threshold) were evaluated in acute visceral hypersensitivity rats induced by restraint stress and colonic hypersensitized rats induced by acetic acid during the neonatal stage assessed by the EMG, AWR, and the spectral analysis of heart rate variability derived from the electrocardiogram.

RESULTS:

1) In normal rats, TNS did not show any effect on the visceromotor reflex. 2) In rats with restraint stress-induced hypersensitivity, TNS with the special set of parameters reduced AWR scores and EMG responses to rectal distention at a pressure of 20-60 mmHg (p < 0.05, vs. baseline for both AWR and EMG). Concurrently, TNS increased vagal activity and decreased sympathetic activity (p < 0.03 for both). 3) Similar effects were noted on the EMG (p < 0.05, vs. baseline) and AWR (p < 0.05 vs. baseline) with acute and chronic TNS in rats with chronic colonic hypersensitivity and the effects were blocked by naloxone.

CONCLUSIONS:

TNS with parameters of 14 Hz, 330 μsec, and 40% motor threshold is effective in improving visceral hypersensitivity in rodent models of colonic hypersensitivity via the modulation of autonomic and opioid mechanisms.

• Authors: Liuqin Jiang, Shengai Zhang, Nina Zhang, Jiande D Z Chen
• Journal: Neuromodulation : journal of the International Neuromodulation Society (Neuromodulation) Vol. 24 Issue 6 Pg. 1003-1011 (Aug 2021) ISSN: 1525-1403 [Electronic] United States
• PMID: 33615647 (Publication Type: Journal Article)
• Copyright: © 2021 International Neuromodulation Society.

Chemical References

• Analgesics, Opioid
• Naloxone

Topics

• Analgesics, Opioid
• Animals
• Colon
• Disease Models, Animal
• Naloxone
• Rats
• Rats, Sprague-Dawley
• Tibial Nerve