Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays.

Abstract

The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

• Authors: Min-Xia Zhang, Wan-Yi Zhao, Qing-Qing Fang, Xiao-Feng Wang, Chun-Ye Chen, Bang-Hui Shi, Bin Zheng, Shou-Jie Wang, Wei-Qiang Tan, Li-Hong Wu
• Journal: Journal of applied biomaterials & functional materials (J Appl Biomater Funct Mater) 2021 Jan-Dec Vol. 19 Pg. 2280800021989698 ISSN: 2280-8000 [Electronic] United States
• PMID: 33560909 (Publication Type: Journal Article)

Chemical References

• Collagen
• Chitosan

Topics

• Animals
• Bandages
• Chitosan (pharmacology)
• Collagen
• Escherichia coli
• Mice
• NIH 3T3 Cells
• Staphylococcus aureus
• Wound Healing