Biosynthesis of sialosyllactotetraosylceramide in human colorectal carcinoma cells.

Abstract

A monosialoganglioside, IV3-NeuNAcLcOse4Cer, has recently been detected in colorectal carcinoma cells, small cell lung carcinoma cells, embryonal carcinoma cells and in human brain extracts. We report here the presence of a CMP-sialic: LcOse4Cer sialyl transferase activity in subcellular membrane fractions of the human colorectal carcinoma. SW1116, which recognizes the non-reducing terminal galactosyl moiety of lactotetraosylceramide. A convenient method for structural analysis of picomolar quantities of the radioactive enzymatic product(s) using bacterial endoglycoceramidase, sialidase and a viral sialidase is presented.

• Authors: A Jolif, V Liepkans
• Journal: FEBS letters (FEBS Lett) Vol. 230 Issue 1-2 Pg. 147-50 (Mar 28 1988) ISSN: 0014-5793 [Print] England
• PMID: 3350149 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Globosides
• Glycosphingolipids
• Sialic Acids
• sialosylgloboside
• Sialyltransferases
• Neuraminidase
• N-Acetylneuraminic Acid

Topics

• Antigens, Neoplasm
• Colonic Neoplasms (metabolism)
• Globosides (biosynthesis)
• Glycosphingolipids (biosynthesis)
• Humans
• N-Acetylneuraminic Acid
• Neuraminidase (metabolism)
• Rectal Neoplasms (metabolism)
• Sialic Acids (metabolism)
• Sialyltransferases (metabolism)
• Tumor Cells, Cultured