Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts.

Abstract

Soluble extracts from human lymphoid cell lines that perform repair synthesis on covalently closed circular DNA containing pyrimidine dimers or psoralen adducts are described. Short patches of nucleotides are introduced by excision repair of damaged DNA in an ATP-dependent reaction. Extracts from xeroderma pigmentosum cell lines fail to act on damaged circular DNA, but are proficient in repair synthesis of ultraviolet-irradiated DNA containing incisions generated by Micrococcus luteus pyrimidine dimer-DNA glycosylase. Repair is defective in extracts from all xeroderma pigmentosum cell lines investigated, representing the genetic complementation groups A, B, C, D, H, and V. Mixing of cell extracts of group A and C origin leads to reconstitution of the DNA repair activity.

• Authors: R D Wood, P Robins, T Lindahl
• Journal: Cell (Cell) Vol. 53 Issue 1 Pg. 97-106 (Apr 08 1988) ISSN: 0092-8674 [Print] United States
• PMID: 3349527 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Adenosine Triphosphate

Topics

• Adenosine Triphosphate (metabolism)
• Cell Line
• Cell-Free System
• DNA Repair
• DNA Replication
• Genetic Complementation Test
• Humans
• Plasmids
• Ultraviolet Rays
• Xeroderma Pigmentosum (genetics)