The effect of
beta-cyclodextrin-benzaldehyde (
CDBA) on the pulmonary
metastasis in C3H/He mice was examined. When mice were treated daily with
CDBA, 3 weeks later the number of lung nodules developed after i.v. inoculation of 1 X 10(6) RCT (+) cells was significantly decreased. The mean numbers of the lung nodules were 69.9 and 73.4 in the water-and
cyclodextrin (CD)-treated mice, respectively. However, these were 17.8, 9.8 and 2.9 in 0.5, 5 and 25 mg/mouse per day
CDBA-treated mice, respectively. And also, daily treatment of
CDBA prolonged the survival time of the
tumor bearing mice in both experimental and spontaneous
metastasis studies. Two or three weeks after subcutaneous inoculation of RCT (+) cells (1 X 10(6) cells) to the foot pad, left hindlimbs were amputated and then mice were daily treated or untreated with
CDBA. Five weeks after
tumor inoculation, the number of lung nodules was counted. Twenty eight point six and 100% of untreated mice had lung
metastases when
amputation was carried out 2 (earlier operation group) and 3 (latero peration group) weeks after
tumor inoculation. However, in
CDBA-treated mice, these values were noticeably decreased, that is, 6.7% and 60% in earlier and later operation groups, respectively. Furthermore, in the later operation group, mean number of the lung nodules in
CDBA-treated mice was only 2.7 while this was 12.9 in untreated mice. These data suggest that
CDBA improve the survival time of
tumor bearing mice through the inhibition of the lung
metastasis.