Protein liposomes-mediated targeted acetylcholinesterase gene delivery for effective liver cancer therapy.

Abstract

BACKGROUND:

Effective methods to deliver therapeutic genes to solid tumors and improve their bioavailability are the main challenges of current medical research on gene therapy. The development of efficient non-viral gene vector with tumor-targeting has very important application value in the field of cancer therapy. Proteolipid integrated with tumor-targeting potential of functional protein and excellent gene delivery performance has shown potential for targeted gene therapy.

RESULTS:

Herein, we prepared transferrin-modified liposomes (Tf-PL) for the targeted delivery of acetylcholinesterase (AChE) therapeutic gene to liver cancer. We found that the derived Tf-PL/AChE liposomes exhibited much higher transfection efficiency than the commercial product Lipo 2000 and shown premium targeting efficacy to liver cancer SMMC-7721 cells in vitro. In vivo, the Tf-PL/AChE could effectively target liver cancer, and significantly inhibit the growth of liver cancer xenografts grafted in nude mice by subcutaneous administration.

CONCLUSIONS:

This study proposed a transferrin-modified proteolipid-mediated gene delivery strategy for targeted liver cancer treatment, which has a promising potential for precise personalized cancer therapy.

• Authors: Kai Wang, Fusheng Shang, Dagui Chen, Tieliu Cao, Xiaowei Wang, Jianpeng Jiao, Shengli He, Xiaofei Liang
• Journal: Journal of nanobiotechnology (J Nanobiotechnology) Vol. 19 Issue 1 Pg. 31 (Jan 22 2021) ISSN: 1477-3155 [Electronic] England
• PMID: 33482834 (Publication Type: Journal Article)

Chemical References

• Liposomes
• Transferrin
• Acetylcholinesterase

Topics

• Acetylcholinesterase (genetics)
• Animals
• Cell Line, Tumor
• Female
• Gene Transfer Techniques
• Genetic Therapy
• Humans
• Liposomes (chemistry)
• Liver Neoplasms (genetics, therapy)
• Mice, Inbred BALB C
• Mice, Nude
• Plasmids (administration & dosage, genetics, therapeutic use)
• Transfection
• Transferrin (chemistry)