The identification of novel immunogenic antigens as potential Shigella vaccine components.

Abstract

BACKGROUND:

Shigella is a major diarrheal pathogen for which there is presently no vaccine. Whole genome sequencing provides the ability to predict and derive novel antigens for use as vaccines. Here, we aimed to identify novel immunogenic Shigella antigens that could serve as Shigella vaccine candidates, either alone, or when conjugated to Shigella O-antigen.

METHODS:

Using a reverse vaccinology approach, where genomic analysis informed the Shigella immunome via an antigen microarray, we aimed to identify novel immunogenic Shigella antigens. A core genome analysis of Shigella species, pathogenic and non-pathogenic Escherichia coli, led to the selection of 234 predicted immunogenic Shigella antigens. These antigens were expressed and probed with acute and convalescent serum from microbiologically confirmed Shigella infections.

RESULTS:

Several Shigella antigens displayed IgG and IgA seroconversion, with no difference in sero-reactivity across by sex or age. IgG sero-reactivity to key Shigella antigens was observed at birth, indicating transplacental antibody transfer. Six antigens (FepA, EmrK, FhuA, MdtA, NlpB, and CjrA) were identified in in vivo testing as capable of producing binding IgG and complement-mediated bactericidal antibody.

CONCLUSIONS:

These findings provide six novel immunogenic Shigella proteins that could serve as candidate vaccine antigens, species-specific carrier proteins, or targeted adjuvants.

• Authors: Ruklanthi de Alwis, Li Liang, Omid Taghavian, Emma Werner, Hao Chung The, Trang Nguyen Hoang Thu, Vu Thuy Duong, D Huw Davies, Philip L Felgner, Stephen Baker
• Journal: Genome medicine (Genome Med) Vol. 13 Issue 1 Pg. 8 (01 15 2021) ISSN: 1756-994X [Electronic] England
• PMID: 33451348 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• Immunoglobulin G
• Shigella Vaccines

Topics

• Antibody Formation (immunology)
• Antigens, Bacterial (immunology)
• Bacterial Proteins (immunology)
• Computational Biology
• Dysentery, Bacillary (blood, immunology, microbiology)
• Fetal Blood (immunology)
• Genome, Human
• Humans
• Immunization
• Immunoglobulin G (blood)
• Seroconversion
• Shigella Vaccines (immunology)