Albumin-based fluorescence resonance energy transfer nanoprobes for multileveled tumor tissue imaging and dye release imaging.

Abstract

Tumor tissue imaging and drug release imaging are both crucial for tumor imaging and image-guided drug delivery. It is urgent to develop a multileveled tumor imaging platform to realize the multiple imaging applications. In this work, we synthesized an albumin-based fluorescence resonance energy transfer (FRET) probe Cy5/7@HSA NPs containing two near-infrared cyanine dyes (CyBI5 and CyBI7) with high FRET efficiency (97 %). Excellent brightness and efficient FRET inside Cy5/7@HSA NPs enabled high-sensitive cell imaging and tumor imaging. This unique nanoprobe imaged 4T1 tumor-bearing mice with high sensitivity (TBR = 5.2) at 24 h post-injection and the dyes penetrated the tumor interior around 4 h post-injection. The release of dyes from nanoprobes was also tracked. This result shows the strong potential of this albumin-based FRET nanoprobe as multileveled tumor imaging platform for in vivo tumor imaging, drug delivery and image-guided surgery.

• Authors: Xianghan Zhang, Xiaohan Gao, Jialin Zhou, Zhiqing Gao, Yingdi Tang, Zuhong Tian, Pengbo Ning, Yuqiong Xia
• Journal: Colloids and surfaces. B, Biointerfaces (Colloids Surf B Biointerfaces) Vol. 199 Pg. 111537 (Mar 2021) ISSN: 1873-4367 [Electronic] Netherlands
• PMID: 33385821 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Albumins
• Fluorescent Dyes

Topics

• Albumins
• Animals
• Fluorescence Resonance Energy Transfer
• Fluorescent Dyes
• Mice
• Neoplasms (diagnostic imaging)
• Optical Imaging