Left Pulmonary Artery Ligation and Chronic Pulmonary Artery Banding Model for Inducing Right Ventricular-Pulmonary Hypertension in Sheep.

Abstract

Pulmonary hypertension (PH) is a progressive disease that leads to cardiopulmonary dysfunction and right heart failure from pressure and volume overloading of the right ventricle (RV). Mechanical cardiopulmonary support has theoretical promise as a bridge to organ transplant or destination therapy for these patients. Solving the challenges of mechanical cardiopulmonary support for PH and RV failure requires its testing in a physiologically relevant animal model. Previous PH models in large animals have used pulmonary bead embolization, which elicits unpredictable inflammatory responses and has a high mortality rate. We describe a step-by-step guide for inducing pulmonary hypertension and right ventricular hypertrophy (PH-RVH) in sheep by left pulmonary artery (LPA) ligation combined with progressive main pulmonary artery (MPA) banding. This approach provides a controlled method to regulate RV afterload as tolerated by the animal to achieve PH-RVH, while reducing acute mortality. This animal model can facilitate evaluation of mechanical support devices for PH and RV failure.

• Authors: Rei Ukita, Yuliya Tipograf, Andrew Tumen, Rachel Donocoff, John W Stokes, Neal M Foley, Jennifer Talackine, Nancy L Cardwell, Erika B Rosenzweig, Keith E Cook, Matthew Bacchetta
• Journal: ASAIO journal (American Society for Artificial Internal Organs : 1992) (ASAIO J) Vol. 67 Issue 1 Pg. e44-e48 (01 01 2021) ISSN: 1538-943X [Electronic] United States
• PMID: 33346995 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © ASAIO 2020.

Topics

• Animals
• Disease Models, Animal
• Hypertension, Pulmonary (physiopathology)
• Hypertrophy, Right Ventricular (physiopathology)
• Ligation
• Male
• Pulmonary Artery (physiopathology, surgery)
• Sheep
• Ventricular Dysfunction, Right (physiopathology)