Metabolic reprogramming of non-
cancer cells residing in a tumor microenvironment, as a result of the adaptations to
cancer-derived metabolic and non-metabolic factors, is an emerging aspect of
cancer-host interaction. We show that in normal and cancer-associated fibroblasts,
breast cancer-secreted extracellular vesicles suppress mTOR signaling upon
amino acid stimulation to globally reduce mRNA translation. This is through delivery of
cancer-derived miR-105 and miR-204, which target RAGC, a component of Rag
GTPases that regulate
mTORC1 signaling. Following
amino acid starvation and subsequent re-feeding, 13 C-
arginine labeling of de novo synthesized
proteins shows selective translation of
proteins that cluster to specific cellular functional pathways. The repertoire of these newly synthesized
proteins is altered in fibroblasts treated with
cancer-derived extracellular vesicles, in addition to the overall suppressed
protein synthesis. In human
breast tumors, RAGC
protein levels are inversely correlated with miR-105 in the stroma. Our results suggest that through educating fibroblasts to reduce and re-prioritize mRNA translation,
cancer cells rewire the metabolic fluxes of
amino acid pool and dynamically regulate stroma-produced
proteins during periodic nutrient fluctuations.