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Preservation of skin by refrigeration for autogenous grafting in the horse.

Abstract
Eighteen stored split thickness meshed skin grafts were applied to surgically created lesions on the metacarpal and metatarsal regions of six horses. Donor skin was harvested from the sternal region, meshed and stored at 4 degrees C in a cell culture medium containing 10% serum. Stored grafts were applied to the wounds at 1, 2, and 3 week intervals. Acceptance of the grafts stored for 1 week was generally poor (1 of 6 grafts), whereas that of the 2 and 3 week old grafts was generally excellent (10 of 12 grafts). Poor acceptance of the 1 week old grafts was attributed to streptococcal infection of the recipient wounds. Using the storage medium and grafting technique described, excellent acceptance can be expected after graft storage of up to 3 weeks.
AuthorsJ Schumacher, M Chambers, D V Hanselka, L D Morton
JournalVeterinary surgery : VS (Vet Surg) Vol. 16 Issue 5 Pg. 358-61 ( 1987) ISSN: 0161-3499 [Print] United States
PMID3333709 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Culture Media
Topics
  • Animals
  • Cold Temperature
  • Culture Media
  • Female
  • Horses (surgery)
  • Male
  • Preservation, Biological
  • Skin Transplantation
  • Time Factors
  • Transplantation, Autologous

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