Abstract |
Balb/c x DBA/2 F1 (CD2F1) mice were lethally irradiated (TBI) and reconstituted with syngeneic bone marrow cells (SBMT) untreated or treated with mafosfamide ( ASTA Z 7654) for ex vivo purging of semisyngeneic L1210 leukemia (TBI + SBMT or TBI + SBMT-Maf mice, respectively). At various times after irradiation and reconstitution mice were injected intraperitoneally four times at weekly intervals with 10(6) immunogenic L1210-Maf cells (L1210 cells treated in vitro with mafosfamide for inhibition of their growth in vivo). As positive controls we immunized normal (non-irradiated) CD2F1 mice. Full resistance against L1210 leukemia (as compared to normal immunized mice) could be obtained in TBI + SBMT and TBI + SBMT-Maf mice when the immunization procedure was started from day +28 or day +56 after transplantation, respectively. Earlier immunization of TBI + SBMT mice (from day +14) or TBI + SBMT-Maf mice (from day +14 or +28) caused only partial resistance against the leukemia.
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Authors | T Skórski, M Kawalec |
Journal | Bone marrow transplantation
(Bone Marrow Transplant)
Vol. 2
Issue 4
Pg. 435-40
(Dec 1987)
ISSN: 0268-3369 [Print] England |
PMID | 3332191
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- mafosfamide
- Cyclophosphamide
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Topics |
- Animals
- Bone Marrow
(pathology)
- Bone Marrow Transplantation
- Cyclophosphamide
(analogs & derivatives, therapeutic use)
- Immunization
- Leukemia L1210
(immunology, pathology, therapy)
- Mice
- Whole-Body Irradiation
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