Anthocyanins Derived from Vitis coignetiae Pulliat Contributes Anti-Cancer Effects by Suppressing NF-κB Pathways in Hep3B Human Hepatocellular Carcinoma Cells and In Vivo.

Abstract

We previously demonstrated that anthocyanins from the fruits of Vitis coignetiae Pulliat (AIMs) induced the apoptosis of hepatocellular carcinoma cells. However, many researchers argued that the concentrations of AIMs were too high for in vivo experiments. Therefore, we performed in vitro at lower concentrations and in vivo experiments for the anti-cancer effects of AIMs. AIMs inhibited the cell proliferation of Hep3B cells in a dose-dependent manner with a maximum concentration of 100 µg/mL. AIMs also inhibited the invasion and migration at 100 µg/mL concentration with or without the presence of TNF-α. To establish the relevance between the in vitro and in vivo results, we validated their effects in a Xenograft model of Hep3B human hepatocellular carcinoma cells. In the in vivo test, AIMs inhibited the tumorigenicity of Hep3B cells in the xenograft mouse model without showing any clinical signs of toxicity or any changes in the body weight of mice. AIMs inhibited the activation NF-κB and suppressed the NF-κB-regulated proteins, intra-tumoral microvessel density (IMVD) and the Ki67 activity of Hep3B xenograft tumors in athymic nude mice. In conclusion, this study indicates that AIMs have anti-cancer effects (inhibition of proliferation, invasion, and angiogenesis) on human hepatocellular carcinoma xenograft through the inhibition of NF-κB and its target protein.

• Authors: Min Jeong Kim, Anjugam Paramanantham, Won Sup Lee, Jeong Won Yun, Seong Hwan Chang, Dong Chul Kim, Hyeon Soo Park, Yung Hyun Choi, Gon Sup Kim, Chung Ho Ryu, Sung Chul Shin, Soon Chan Hong
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 25 Issue 22 (Nov 20 2020) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 33233701 (Publication Type: Journal Article)

Chemical References

• Anthocyanins
• Antineoplastic Agents, Phytogenic
• Biomarkers
• NF-kappa B
• Plant Extracts
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Animals
• Anthocyanins (chemistry, pharmacology)
• Antineoplastic Agents, Phytogenic (chemistry, pharmacology)
• Biomarkers
• Carcinoma, Hepatocellular (drug therapy, genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Disease Models, Animal
• Humans
• Liver Neoplasms (drug therapy, genetics, metabolism, pathology)
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Mice
• Mice, Nude
• NF-kappa B (metabolism)
• Plant Extracts (chemistry, pharmacology)
• Signal Transduction (drug effects)
• Vitis (chemistry)
• Xenograft Model Antitumor Assays