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Studies on induced Klebsiella mastitis with relationships among N-acetyl-beta-D-glucosaminidase, bacterial and somatic cell counts.

AbstractMastitis was induced by infusion of 3,000-7,000 colony-forming units of Klebsiella pneumoniae into the left rear quarter of four primiparous Holstein-Freisians. Greater than 100 organisms were recovered from a quarter milk sample within 2 hr and in 87.5% of the hourly cultures taken after 8 hr. Bacteria, California Mastitis Tests, somatic cell counts and N-acetyl-beta-D-glucosaminidase levels differed significantly from that of control quarters in infused cows after hr 6, 8, 9 and 9 respectively. High correlations were obtained between somatic cell count and N-acetyl-beta-D-glucosaminidase (r = .97) and N-acetyl-beta-D-glucosaminidase and natural log bacterial count (r. = .88). N-acetyl-beta-D-glucosaminidase was related to natural log bacterial counts of samples taken 1, 2, 3, and 4 hr prior to the test (rN-acetyl-beta-D-glucosaminidase and natural log bacterial count (r = .91, .92, .93, and .95).
AuthorsJ R Kunkel, R B Bushnell, J Cullor, J Aleong (Affiliation: Department of Animal Science, University of Vermont, Burlington 05405.)
JournalThe Cornell veterinarian (Cornell Vet) Vol. 77 Issue 3 Pg. 225-34 (Jul 1987) ISSN: 0010-8901 UNITED STATES
PMID3315443 (Publication Type: Journal Article)
Chemical References
  • Hexosaminidases
  • Acetylglucosaminidase
Topics
  • Acetylglucosaminidase (analysis)
  • Animals
  • Cattle
  • Cell Count
  • Female
  • Hexosaminidases (analysis)
  • Klebsiella Infections (enzymology, pathology, veterinary)
  • Klebsiella pneumoniae
  • Mastitis, Bovine (enzymology, microbiology, pathology)
  • Milk (analysis, cytology, enzymology, microbiology)