Studies on induced Klebsiella mastitis with relationships among N-acetyl-beta-D-glucosaminidase, bacterial and somatic cell counts.

Abstract

Mastitis was induced by infusion of 3,000-7,000 colony-forming units of Klebsiella pneumoniae into the left rear quarter of four primiparous Holstein-Freisians. Greater than 100 organisms were recovered from a quarter milk sample within 2 hr and in 87.5% of the hourly cultures taken after 8 hr. Bacteria, California Mastitis Tests, somatic cell counts and N-acetyl-beta-D-glucosaminidase levels differed significantly from that of control quarters in infused cows after hr 6, 8, 9 and 9 respectively. High correlations were obtained between somatic cell count and N-acetyl-beta-D-glucosaminidase (r = .97) and N-acetyl-beta-D-glucosaminidase and natural log bacterial count (r. = .88). N-acetyl-beta-D-glucosaminidase was related to natural log bacterial counts of samples taken 1, 2, 3, and 4 hr prior to the test (rN-acetyl-beta-D-glucosaminidase and natural log bacterial count (r = .91, .92, .93, and .95).

• Authors: J R Kunkel, R B Bushnell, J Cullor, J Aleong
• Journal: The Cornell veterinarian (Cornell Vet) Vol. 77 Issue 3 Pg. 225-34 (Jul 1987) ISSN: 0010-8901 [Print] United States
• PMID: 3315443 (Publication Type: Journal Article)

Chemical References

• Hexosaminidases
• Acetylglucosaminidase

Topics

• Acetylglucosaminidase (analysis)
• Animals
• Cattle
• Cell Count
• Female
• Hexosaminidases (analysis)
• Klebsiella Infections (enzymology, pathology, veterinary)
• Klebsiella pneumoniae
• Mastitis, Bovine (enzymology, microbiology, pathology)
• Milk (analysis, cytology, enzymology, microbiology)