Abstract | OBJECTIVE: Recent studies have focused on the special roles of NADPH-oxidase in multiple autoimmune diseases. Nevertheless, the association of genetic variation in NADPH-oxidase genes with rheumatoid arthritis (RA) was not extensively studied in a Chinese population. We performed this study to examine the association of NCF2, NCF4, and CYBA gene polymorphisms with RA susceptibility in a Chinese population. METHODS: Six single nucleotide polymorphisms (SNPs) (NCF2 rs10911363, NCF4 rs1883112, rs4821544, rs729749, CYBA rs3794624, and rs4673) were genotyped in a cohort composed of 593 RA patients and 596 normal controls. Improved multiple ligase detection reaction (iMLDR) was used for genotyping. RESULTS: We observed that NCF4 rs4821544 CT genotype and C allele frequencies in RA patients were significantly decreased when compared to controls (CT vs. TT: P = 0.043; C vs. T: P = 0.031), and rs4821544 polymorphism was significantly associated with an increased RA risk under the dominant model (TT vs. CT+CC: P = 0.031). Our results also indicated that rs729749 CT genotype frequency was significantly lower in RA patients than that in controls (CT vs. CC: P = 0.033). Moreover, the rs729749 CT genotype frequency was also significantly decreased in RA patients in males (CT vs. CC: P = 0.024). No significant association between NCF2 and CYBA gene polymorphisms and RA susceptibility was observed. There were significant associations between rs4821544 TT genotype and T allele frequencies and anti-CCP in male RA patients. CONCLUSIONS: In summary, NCF4 rs4821544 and rs729749 polymorphisms might contribute to RA susceptibility, while NCF2 and CYBA gene polymorphisms were not associated with RA susceptibility.
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Authors | Tian-Ping Zhang, Rui Li, Qian Huang, Han-Feng Pan, Dong-Qing Ye, Xiao-Mei Li |
Journal | Journal of immunology research
(J Immunol Res)
Vol. 2020
Pg. 8528976
( 2020)
ISSN: 2314-7156 [Electronic] Egypt |
PMID | 33145364
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Tian-Ping Zhang et al. |
Chemical References |
- NADPH Oxidases
- CYBA protein, human
- NCF2 protein, human
- NCF4 protein, human
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Topics |
- Aged
- Arthritis, Rheumatoid
(genetics)
- Case-Control Studies
- China
- Cohort Studies
- Female
- Gene Frequency
- Genetic Predisposition to Disease
- Genotype
- Humans
- Male
- Middle Aged
- NADPH Oxidases
(genetics)
- Polymorphism, Single Nucleotide
- Risk
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