Abstract |
Four cases of acute myelofibrosis are contrasted to three cases of acute megakaryocytic leukemia. The cases were clinically indistinguishable. Light microscopic examination of hematoxylin and eosin-stained bone marrow biopsy specimens from the cases of acute myelofibrosis showed a trilinear proliferation of mature and immature hematopoietic cells, increased numbers of mature magakaryocytes, and marked fibrosis. In contrast, the blast cells in the cases of acute megakaryocytic leukemia showed a continuous spectrum of differentiation from small blasts to megakaryoblasts. Immunoperoxidase staining showed the blast cells in all three cases of acute megakaryocytic leukemia to be strongly positive for Factor VIII, a marker of megakaryocytic differentiation. These same blasts did not stain with markers of myelomonocytic ( lysozyme), lymphocytic (Hle), and myeloid (Leu-Ml) differentiation. In contrast, the blast cells in all four cases of acute myelofibrosis were Factor VIII, Hle, and Leu-Ml negative but did occasionally stain with anti- lysozyme.
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Authors | R H Hruban, F P Kuhajda, R B Mann |
Journal | American journal of clinical pathology
(Am J Clin Pathol)
Vol. 88
Issue 5
Pg. 578-88
(Nov 1987)
ISSN: 0002-9173 [Print] England |
PMID | 3314470
(Publication Type: Case Reports, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Acute Disease
- Adult
- Blood Cells
(pathology)
- Bone Marrow
(pathology)
- Female
- Humans
- Immunoenzyme Techniques
- Male
- Middle Aged
- Primary Myelofibrosis
(blood, immunology, pathology)
- Thrombocythemia, Essential
(immunology, pathology)
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