Abstract | OBJECTIVE: MATERIALS AND METHODS: We recruited patients with MG who had not received prednisone or immunosuppressive therapies between June 2015 and June 2019, and divided them into AOMG and JOMG subgroups according to their ages at the time of recruitment. Clinical information and blood samples were collected. Serum AChR antibodies were detected by CBAs in HEK293T cells expressing clustered adult and fetal AChRs, as well as by enzyme-linked immunosorbent assays (ELISAs). Differences in AChR antibody profiles between AOMG and JOMG subgroups were determined. RESULTS: A total of 239 patients with MG were enrolled in the present study, including 121 AOMG and 118 JOMG patients. Based on ELISAs, 74.4% of AOMG (90/121) and 59.3% of JOMG (70/118) patients were anti-AChR positive (p = 0.02). However, CBAs yielded equal anti-AChR positivities (p = 0.64), as indicated by 80.2% of AOMG patients (97/121) and 77.1% of JOMG patients (91/118). Furthermore, among AOMG patients, 67.8% (82/121) were positive for both adult and fetal AChR antibodies, 5.8% (7/121) were positive for only adult AChR antibodies, and 6.6% (8/121) were positive for only fetal AChR antibodies, while these rates were 50.8% (60/118), 21.2% (25/118), and 5.1% (6/118), respectively, in JOMG cohorts (p < 0.01). Twenty-nine AOMG patients and 10 JOMG patients underwent IgG subclassification of AChR antibodies, which were all confirmed to be predominantly IgG1. CONCLUSIONS: The positive rates of AChR antibodies did not differ between Chinese AOMG and JOMG patients, as revealed by CBAs. Furthermore, the screened AChR antibodies were predominantly IgG1 in both AOMG and JOMG patients.
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Authors | Chong Yan, Rui Zhao, Jie Song, Xuelin Feng, Jianying Xi, Sushan Luo, Huahua Zhong, Shuizhen Zhou, Wenhui Li, Chongbo Zhao |
Journal | Journal of neuroimmunology
(J Neuroimmunol)
Vol. 349
Pg. 577403
(12 15 2020)
ISSN: 1872-8421 [Electronic] Netherlands |
PMID | 32992216
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020. Published by Elsevier B.V. |
Chemical References |
- Autoantibodies
- Biomarkers
- Immunoglobulin G
- Receptors, Cholinergic
|
Topics |
- Adolescent
- Adult
- Age Factors
- Aged
- Autoantibodies
(blood)
- Biomarkers
(blood)
- Child
- Child, Preschool
- China
(epidemiology)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Female
- HEK293 Cells
- Humans
- Immunoglobulin G
(blood)
- Infant
- Male
- Middle Aged
- Myasthenia Gravis
(blood, diagnosis, epidemiology)
- Receptors, Cholinergic
(blood)
- Young Adult
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