Protective immunity evoked by oral administration of attenuated aroA Salmonella typhimurium expressing cloned streptococcal M protein.

Abstract	Attenuated strains of Salmonella have been used effectively as vaccines against typhoid fever. We have investigated the use of such strains to deliver cloned antiphagocytic virulence determinants of unrelated bacteria. The aroA strain of S. typhimurium SL3261 was transformed with a low-copy plasmid vector pMK207, which contains the cloned gene spm5 encoding streptococcal M protein, the major virulence factor of these organisms. The transformed SL3261 expressed type 5 M protein in the cytoplasmic fraction, and when fed orally to BALB/c mice, evoked both serum and salivary IgA, IgG, and IgM antibodies directed against type 5 M protein. The orally immunized mice were completely protected against both intranasal and intraperitoneal challenge infections with virulent S. typhimurium SL1344 or M5 streptococci. These studies provide evidence that an attenuated strain of Salmonella can be used effectively as a general vaccine vehicle to deliver antiphagocytic virulence determinants of unrelated bacteria.
Authors	T P Poirier, M A Kehoe, E H Beachey
Journal	The Journal of experimental medicine (J Exp Med) Vol. 168 Issue 1 Pg. 25-32 (Jul 01 1988) ISSN: 0022-1007 [Print] United States
PMID	3294331 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References	Antigens Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Carrier Proteins DNA, Recombinant Immunoglobulin A Immunoglobulin G Immunoglobulin M Vaccines, Attenuated Vaccines, Synthetic streptococcal M protein
Topics	Animals Antigens (immunology) Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins (genetics, immunology) Carrier Proteins DNA, Recombinant Enzyme-Linked Immunosorbent Assay Immunization Immunoglobulin A (analysis) Immunoglobulin G (analysis) Immunoglobulin M (analysis) Mice Mice, Inbred BALB C Salmonella Infections, Animal (prevention & control) Salmonella typhimurium (immunology) Streptococcal Infections (prevention & control) Transformation, Bacterial Vaccines, Attenuated (immunology) Vaccines, Synthetic (immunology)

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