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The mitochondria/caspase-dependent apoptotic pathway plays a role in the positive effects of a power frequency electromagnetic field on Alzheimer's disease neuronal model.

Abstract
In this study, rat pheochromocytoma (PC12) cells were induced into an Alzheimer's Disease (AD) neuronal model using nerve growth factor (NGF; 50 ng/mL) and Amyloid β25-35 (20 μmol/L). Changes in the morphological structure, cell viability, apoptosis rate, and expression of apoptosis-related protein induced by exposure to a power frequency electromagnetic field (PF-MF; 50 Hz, 100 μT, 24 h) were detected respectively by light and electron microscopy, the MTT assay, immunohistochemistry, flow cytometry and enzyme-linked immunosorbent assays. The results showed that 3-12 h after PF-MF exposure, the pathological injury was improved partly; metabolic activity was promoted and cell apoptosis was inhibited in the AD neuronal model. In addition, PF-MF exposure significantly inhibited the expression of Caspase8, Caspase3, and CytC, but increased the Bcl-2/Bax ratio of the AD neuronal model. Meanwhile, PF-MF seemed to have no effect on the expression of Fas and TNFR1. This study indicated that the mitochondria/caspase-dependent apoptotic pathway plays an important role in the positive effects of PF-MF on an AD neuronal model. The results suggested that PF-MF exposure might have potential therapeutic value for AD, and the underling molecular mechanisms still need further studies.
AuthorsHongyan Zuo, Xiao Liu, Yang Li, Dewen Wang, Yanhui Hao, Chao Yu, Xinping Xu, Ruiyun Peng, Tao Song
JournalJournal of chemical neuroanatomy (J Chem Neuroanat) Vol. 109 Pg. 101857 (11 2020) ISSN: 1873-6300 [Electronic] Netherlands
PMID32918997 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2020 Elsevier B.V. All rights reserved.
Chemical References
  • Nerve Growth Factor
  • Caspases
Topics
  • Alzheimer Disease (metabolism)
  • Animals
  • Apoptosis (physiology, radiation effects)
  • Caspases (metabolism)
  • Cell Differentiation (drug effects)
  • Cell Survival (physiology, radiation effects)
  • Electromagnetic Fields
  • Mitochondria (metabolism, radiation effects)
  • Nerve Growth Factor (pharmacology)
  • Neurons (drug effects, metabolism, radiation effects)
  • PC12 Cells
  • Rats

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