Gastric cancer is one of the most frequently diagnosed
cancer and poses a serious threat to health system in the world. Upregulation of
meningioma-associated
protein (MAC30) has been found in many solid
tumors and can regulate the proliferation, differentiation, and apoptosis of different
tumor cells. Quantitative polymerase chain reaction (qPCR) was used to detect the expression of MAC30 in 68 patients with
gastric cancer and their adjacent tissues. Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into
gastric cancer BGC-823 cell line and the expression of lentivirus label
protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively. Also, related
protein expressions on Wnt/β-
catenin signaling pathway were analyzed by Western blot method. The expression of MAC30 was abnormally elevated in
gastric cancer tissues, while interfering of its expression could significantly inhibit the proliferation of
gastric cancer BGC-823 cell line. However, the promotion of apoptosis by mitochondrial pathway was mediated by Bax/Bcl-2 upregulation. Present work showed the effect of downregulated MAC30 expression on proliferation and apoptosis of
gastric cancer cell through Wnt/β-
catenin signaling pathway. Thus, this investigation provides an experimental basis for future development of chemotherapeutic agent on
gastric cancer.