99m Tc-labeled Duramycin for detecting and monitoring cardiomyocyte death and assessing atorvastatin cardioprotection in acute myocardial infarction.

Abstract

This study aimed to dynamically monitor myocardial cell death using 99m Tc-Duramycin single-photon emission computed tomography/computed tomography (micro-SPECT/CT) imaging in acute myocardial infarction (AMI) and the anti-apoptosis effect of atorvastatin for cardioprotection. Mice were randomized into three groups: AMI group, AMI with atorvastatin treatment (T-AMI) group, and sham group. Three groups of model mice were randomly selected at day 1 (D1), day 3 (D3), and day 7 (D7) day after surgery with 99m Tc-Duramycin micro-SPECT/CT imaging. The lesion-to-normal myocardial tissue ratio (L/N) average values were 2.62 on D1, 3.89 on D3, and 1.20 on D7 for the uptake of 99m Tc-duramycin in the infarcted region in the AMI group. The sham group presented no positive imaging in myocardium, and the L/N average values were 1.09, 1.14, and 1.10 on D1, D3, and D7, respectively. Meanwhile, 99m Tc-linear-duramycin imaging showed no radioactive uptake in the infarction region. The T-AMI group imaging showed tracer uptake decreased obviously compared to the uptake in the infarcted region in AMI mice. 99m Tc-Duramycin SPECT/CT imaging allowed non-invasive monitoring of myocardial cell death in a mouse model of AMI and an assessment of atorvastatin anti-apoptosis effect for cardioprotection by in vivo molecular imaging.

• Authors: Hui Tan, Mieradilijiang Abudupataer, Lin Qiu, Wujian Mao, Jie Xiao, Dengfeng Cheng, Hongcheng Shi
• Journal: Chemical biology & drug design (Chem Biol Drug Des) Vol. 97 Issue 2 Pg. 210-220 (02 2021) ISSN: 1747-0285 [Electronic] England
• PMID: 32881342 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 John Wiley & Sons A/S.

Chemical References

• Bacteriocins
• Cardiotonic Agents
• Peptides
• Radiopharmaceuticals
• Technetium-99
• duramycin
• Technetium
• Atorvastatin

Topics

• Acute Disease
• Animals
• Apoptosis (drug effects)
• Atorvastatin (pharmacology, therapeutic use)
• Bacteriocins (chemistry, pharmacology)
• Cardiotonic Agents (pharmacology, therapeutic use)
• Disease Models, Animal
• Mice
• Myocardial Infarction (diagnostic imaging, drug therapy)
• Myocardium (cytology, metabolism, pathology)
• Peptides (chemistry, pharmacology)
• Radiopharmaceuticals (chemistry, pharmacology)
• Single Photon Emission Computed Tomography Computed Tomography
• Technetium (chemistry)