Solanine inhibits proliferation and promotes apoptosis of the human leukemia cells by targeting the miR-16/Bcl-2 axis.

Abstract

PURPOSE:

Leukemia causes tremendous human mortality especially in children and young adults. This study was undertaken to investigate the anticancer effects of Solanine against the normal human NCI-H526 and human leukemia AML-193 cell lines.

METHODS:

Cell proliferation was determined by MTT assay. DAPI and annexin V/propidium iodide (PI) assays were used for the determination of apoptosis. The expression analysis was done by qRT-PCR. Protein concentrations were determined by western blot analysis.

RESULTS:

DAPI staining showed that Solanine causes nuclear morphological changes. The annexin V/PI staining showed that Solanine increased the leukemia apoptotic cell death dose-dependently. The expression of Bax was increased while of Bcl-2 was decreased. The qRT-PCR analysis showed that microRNA (miR)-16 was significantly (p<0.05) downregulated in leukemia AML-193 cells as compared to normal NCI-H526 cells.

CONCLUSION:

Taken together, these results showed that Solanine inhibits the proliferation of leukemia cells via induction of apoptosis and modulation of miR-16/Bcl-2 axis.

• Authors: Yan Zheng, Lan Li, Qiuying Gao, Ben Niu, Hui Wang
• Journal: Journal of B.U.ON. : official journal of the Balkan Union of Oncology (J BUON) 2020 May-Jun Vol. 25 Issue 3 Pg. 1614-1618 ISSN: 2241-6293 [Electronic] Cyprus
• PMID: 32862612 (Publication Type: Journal Article)

Chemical References

• BCL2 protein, human
• MIRN16 microRNA, human
• MicroRNAs
• Proto-Oncogene Proteins c-bcl-2
• Solanine

Topics

• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Proliferation (drug effects)
• Down-Regulation (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Leukemia (drug therapy, metabolism)
• MicroRNAs (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Solanine (pharmacology)