The key functional molecules involved in
inflammatory bowel disease (IBD) and IBD-induced colorectal
tumorigenesis remain unclear. In this study, we found that the apoptosis repressor with caspase recruitment domain (
ARC)
protein plays critical roles in IBD.
ARC-deficient mice exhibited substantially higher susceptibility to
dextran sulfate sodium (DSS)-induced IBD compared with wild-type mice. The inflammatory burden induced in
ARC-deficient conditions was inversely correlated with CCL5 and CXCL5 levels in immune cells, especially CD4-positive T cells. Pathologically,
ARC expression in immune cells was significantly decreased in clinical biopsy specimens from patients with IBD compared with normal subjects. In addition,
ARC levels inversely correlated with CCL5 and CXCL5 levels in human biopsy specimens.
ARC interacted with
TNF receptor associated factor (TRAF) 6, regulating ubiquitination of
TRAF6, which was associated with NF-κB signaling. Importantly, we identified a novel ubiquitination site at
lysine 461, which was critical in the function of
ARC in IBD.
ARC played a critical role in IBD and IBD-associated
colon cancer in a
bone marrow transplantation model and
azoxymethane/DSS-induced
colitis cancer mouse models. Overall, these findings reveal that
ARC is critically involved in the maintenance of intestinal homeostasis and protection against IBD through its ubiquitination of
TRAF6 and subsequent modulation of NF-κB activation in T cells. SIGNIFICANCE: This study uncovers a crucial role of
ARC in the immune system and IBD, giving rise to a novel strategy for IBD and IBD-associated
colon cancer therapy.