Stevia is a herbaceous plant containing non-
sugar sweeteners that could be regarded as a successor to
sugar for diabetics. However, there are some problems with the cultivation of stevia, especially in the greenhouse, including unbranching and low
sweetening agents. To overcome this issue, an experiment was designed to increase not only the branching and leaf production but also the sweetness. Therefore, a novel strategy using
abscisic acid (0, 50, 100, 150 μM), its inhibitor, i.e.
fluridone (0, 50, 100, 150 μM) and
decapitation of plant apical meristems was applied. Results showed that when stevia was decapitated, dormant buds responded to the application of
abscisic acid and
fluridone. Under these conditions, axillary buds were developed to branches. As well leaf, total dry weight, soluble
sugars and
steviol glycosides (SGs) were significantly increased. In addition, the interaction of
abscisic acid (50 and 100 μM) and
fluridone (50 μM) had the highest positive effects on plant growth and
steviol glycosides production rather than their sole applications. Results also indicated that
decapitation removed a terminal dominance over a limited period of time and the terminal dominance was re-established with the growth of the terminal branches, whereas the influence of 50 µM
fluridone on stevia was long term and the number of shoots was greater. Since stevia is a costly
sweetener, the results of this study could be used in greenhouses, where the cultivation of stevia seems to be reasonable in terms of economic aspects.