In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.

Abstract

Stem-cell therapy has been proved as a promising strategy for myocardial infarction (MI) treatment. However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs). In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation. And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro. The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization. The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis. Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.

• Authors: Long Gao, Min Yi, Min Xing, Hekai Li, Yanling Zhou, Qing Xu, Zhaowenbin Zhang, Zhanpeng Wen, Jiang Chang
• Journal: Journal of materials chemistry. B (J Mater Chem B) Vol. 8 Issue 34 Pg. 7713-7722 (09 14 2020) ISSN: 2050-7518 [Electronic] England
• PMID: 32724972 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydrogels
• Imines
• Polyglutamic Acid
• Chitosan

Topics

• Animals
• Apoptosis (drug effects)
• Cell Communication (drug effects)
• Chitosan (chemistry)
• Glass (chemistry)
• Hydrogels (chemistry, pharmacology)
• Imines (chemistry)
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells (cytology, drug effects)
• Mice
• Myocardial Infarction (pathology, therapy)
• Myocytes, Cardiac (drug effects, pathology)
• Polyglutamic Acid (chemistry)