Abstract | BACKGROUND: Often concomitant patch test (PT) reactivity to palladium (Pd) and nickel (Ni) is found. OBJECTIVES: To determine whether lymphocyte transformation test (LTT) could be useful in discrimination between cross-reacting or distinct PT results, and to compare the results with in vitro cytokine production upon Pd or Ni stimulation. MATERIALS AND METHODS: The study population consisted of two groups: 13 individuals with Pd PT reactions (10 with concomitant Ni PT reaction, 3 individuals with only Pd PT reactivity) and 10 Ni/Pd PT negative individuals. LTT and assessment of cytokine release ( interferon-gamma, interleukin-5 [IL-5], IL-8, IL-17A, tumor necrosis factor alpha) by cytometric bead assay were performed. RESULTS: All 10 patients with positive PT to Ni and Pd showed positive LTT to Ni (P < .05) as compared with the 10 Pd/Ni PT negative patients-but had no significant LTT reaction to Pd. In all, 9 out of 10 Pd/Ni PT negative patients were also LTT negative to Ni and 10 out of 10 to Pd. In the 3 only Pd PT reactors 2 out of 3 remained LTT negative to Ni and 0 out of 3 to Pd. As a major finding, cytokine production gave clearly enhanced IL-5 response to Ni in Ni PT positive individuals (P < .05), whereas Pd PT reactivity was not linked with such enhanced IL-5 production in vitro to Pd. CONCLUSIONS: Pd and Ni sensitization are mostly found concomitantly, and cross-reactivity is questioned. By different LTT reactions and particularly IL-5 production in vitro, predominant Ni sensitization becomes more evident.
|
Authors | Florian Kapp, Burkhard Summer, Peter Thomas |
Journal | Immunity, inflammation and disease
(Immun Inflamm Dis)
Vol. 8
Issue 4
Pg. 483-492
(12 2020)
ISSN: 2050-4527 [Electronic] England |
PMID | 32720755
(Publication Type: Journal Article)
|
Copyright | © 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. |
Chemical References |
- Cytokines
- Palladium
- Nickel
|
Topics |
- Adult
- Aged
- Cytokines
- Female
- Humans
- Hypersensitivity
- Lymphocyte Activation
- Male
- Middle Aged
- Nickel
- Palladium
|