L-2-oxothiazolidine-4-carboxylate protects cultured endothelial cells against hyperoxia-induced injury.

Abstract

When bovine pulmonary artery endothelial monolayers were exposed to hyperoxia (95% O2 and 5% Co2), they responded by selectively elevating the intracellular concentration of glutathione without affecting the activities of glutathione peroxidase or glutathione reductase, L-2-Oxothiazolidine-4-carboxylate, an intracellular cysteine-delivering agent, further enhanced the intracellular concentration of glutathione in oxygen-exposed endothelial cells and protected them from the lethal effect of hyperoxia. In contrast, buthionine sulfoximine, a potent inhibitor of gamma-glutamylcysteine synthetase, reduced the glutathione concentration and rendered the cells more sensitive to the toxic effect of oxygen. Both L-2-oxothiazolidine-4-carboxylate and buthionine sulfoximine had no effect on the activities of glutathione peroxidase or glutathione reductase. Our results suggest that L-2-oxothiazolidine-4-carboxylate may have the potential of preventing oxygen toxicity.

• Authors: M F Tsan, P G Phillips
• Journal: Inflammation (Inflammation) Vol. 12 Issue 2 Pg. 113-21 (Apr 1988) ISSN: 0360-3997 [Print] United States
• PMID: 3270344 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Thiazoles
• Thiazolidines
• Glutathione
• Oxygen
• Pyrrolidonecarboxylic Acid
• 2-oxothiazolidine-4-carboxylic acid

Topics

• Cells, Cultured
• Endothelium, Vascular (drug effects)
• Glutathione (metabolism)
• Oxygen (toxicity)
• Pyrrolidonecarboxylic Acid
• Thiazoles (pharmacology)
• Thiazolidines