Abstract |
Adipose-derived stem cells (ASC) are said to have a pivotal role in wound healing. Specifically, ASC-secreted extracellular vesicles (EV) carry diverse cargos such as microRNAs ( miRNAs) to participate in the ASC-based therapies. Considering its effects, we aimed to investigate the role of ASC-EVs in the cutaneous wound healing accompanied with the study on the specific cargo-medicated effects on wound healing. Two full-thickness excisional skin wounds were created on mouse dorsum, and wound healing was recorded at the indicated time points followed by histological analysis and immunofluorescence staining for CD31 and α-SMA. Human skin fibroblasts (HSFs) and human microvascular endothelial cells (HMECs) were co-cultured with EVs isolated from ASC (ASC-EVs), respectively, followed by the evaluation of their viability and mobility using CCK-8, scratch test and transwell migration assays. Matrigel-based angiogenesis assays were performed to evaluate vessel-like tube formation by HMECs in vitro. ASC-EVs accelerated the healing of full-thickness skin wounds, increased re-epithelialization and reduced scar thickness whilst enhanced collagen synthesis and angiogenesis in murine models. However, miR-486-5p antagomir abrogated the ASC-EVs-induced effects. Intriguingly, miR-486-5p was found to be highly enriched in ASC-EVs, exhibiting an increase in viability and mobility of HSFs and HMECs and enhanced the angiogenic activities of HMECs. Notably, we also demonstrated that ASC-EVs-secreted miR-486-5p achieved the aforesaid effects through its target gene Sp5. Hence, our results suggest that miR-486-5p released by ASC-EVs could be a critical mediator to develop an ASC-based therapeutic strategy for wound healing.
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Authors | Yingjie Lu, Huicai Wen, Jinjun Huang, Peng Liao, Huaiwei Liao, Jun Tu, Yuanlin Zeng |
Journal | Journal of cellular and molecular medicine
(J Cell Mol Med)
Vol. 24
Issue 17
Pg. 9590-9604
(09 2020)
ISSN: 1582-4934 [Electronic] England |
PMID | 32666704
(Publication Type: Journal Article)
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Copyright | © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. |
Chemical References |
- MIRN486 microRNA, human
- MicroRNAs
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Topics |
- Adipocytes
(metabolism, pathology)
- Adipose Tissue
(metabolism, pathology)
- Adult
- Aged
- Cells, Cultured
- Cicatrix
(metabolism, pathology)
- Endothelial Cells
(metabolism, pathology)
- Extracellular Vesicles
(metabolism, pathology)
- Female
- Fibroblasts
(metabolism, pathology)
- Humans
- MicroRNAs
(metabolism)
- Middle Aged
- Neovascularization, Pathologic
(metabolism, pathology)
- Skin
(metabolism, pathology)
- Stem Cells
(metabolism, pathology)
- Wound Healing
(physiology)
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