Anticancer Activity Study and Density Functional/Time-Dependent Density Functional Theory (DFT/TD-DFT) Calculations of 2(3),9(10),16(17),23(24)-Tetrakis-(6-Methylpyridin-2-Yloxy)Phthalocyaninato Zn(II).

Abstract

Photodynamic therapy (PDT) is one of the major therapeutic methods for the treatment of infectious diseases and cancer. Recently, cell culture has been used to determine the effect of a given substance on various pathological conditions, such as cancer. In this study, we aimed to investigate the effect of a Zn- phthalocyanine (ZnPc) derivative on selected cancer cells via a cell culture medium. Methylthiazole tetrazolium (MTT) assay was applied to evaluate the cytotoxic activity of 2(3),9(10),16(17),23(24)-tetrakis-(6-methylpyridin-2-yloxy)phthalocyaninato Zn(II) on rat glioma cells (C6 glioma), human lung cancer cells (H1299) and human umbilical vein endothelial cells (HUVEC). The levels of the lipid peroxidation were determined by measuring the amount of the thiobarbituric acid reactive substance (TBARS) produced using the TBARS assay. The relationship between the oxidative damage and the effective concentration of cytotoxic ZnPc was determined from the results. The apoptotic and genotoxic effects of the phthalocyanine (Pc) were also investigated. Density functional/time-dependent density functional theory (DFT/TD-DFT) methods were used to determine the molecular excited state properties of the ZnPc and chloroaluminum phthalocyanine (ClAlPc) previously reported by Castilho-Fernandes et al. The computed and experimental data were used to establish a link between the electronic and anticancer properties of the Pcs.

• Authors: Işik Didem Karagöz, Yusuf Yilmaz, Kayode Sanusi
• Journal: Journal of fluorescence (J Fluoresc) Vol. 30 Issue 5 Pg. 1151-1160 (Sep 2020) ISSN: 1573-4994 [Electronic] Netherlands
• PMID: 32648171 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Indoles
• Isoindoles
• Organometallic Compounds
• Photosensitizing Agents
• Zinc Compounds
• Zn(II)-phthalocyanine

Topics

• Antineoplastic Agents (chemical synthesis, chemistry, pharmacology)
• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Density Functional Theory
• Drug Screening Assays, Antitumor
• Humans
• Indoles (chemical synthesis, chemistry, pharmacology)
• Isoindoles
• Molecular Structure
• Organometallic Compounds (chemical synthesis, chemistry, pharmacology)
• Photochemotherapy
• Photosensitizing Agents (chemical synthesis, chemistry, pharmacology)
• Time Factors
• Zinc Compounds