Antimyristoylation of gag proteins in human T-cell leukemia and human immunodeficiency viruses with N-myristoyl glycinal diethylacetal.

Abstract

N-Myristoyl (N-Myr-) glycinal (aminoacetoaldehyde, GO) diethylacetal (A), which is abbreviated as N-Myr-GOA, and other N-Myr-compounds (N-Myr-Gly-GOA, N-Myr-Gly-Gly-GOA, and N-Myr-Gly-Gly-Gly-GOA) were newly synthesized and then employed for NH2-terminal antimyristoylation of structure proteins in the human T-cell leukemia virus (HTLV-I) and the human immunodeficiency virus (HIV). The protein myristoylation of structure proteins p19gag, of HTLV-I, and p17gag, of HIV, was determined separately, using radiolabeled myristic acid, in vitro. The radiolabeled proteins, after immunoprecipitation with an antiserum to adult T-cell leukemia (ATL) or the anti-p17gag monoclonal antibody of HIV, were identified as p19gag of HTLV-I and p17gag of HIV by fluorography after SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The protein myristoylation was resistant to NH2OH-treatment. Of the N-Myr-compounds tested, N-Myr-GOA remarkably prevented the myristoylation of p19gag and p17gag, but N-Myr-Gly-Gly-GOA and N-Myr-Gly-Gly-Gly-GOA did not.

• Authors: S Shoji, A Tashiro, Y Kubota
• Journal: Journal of biochemistry (J Biochem) Vol. 103 Issue 5 Pg. 747-9 (May 1988) ISSN: 0021-924X [Print] England
• PMID: 3263365 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetals
• Gene Products, gag
• Myristic Acids
• Retroviridae Proteins
• Myristic Acid
• N-myristoyl glycinal diethylacetal
• Acetaldehyde

Topics

• Acetaldehyde (pharmacology)
• Acetals (pharmacology)
• Gene Products, gag
• HIV (drug effects, metabolism)
• Human T-lymphotropic virus 1 (drug effects, metabolism)
• Myristic Acid
• Myristic Acids (metabolism)
• Retroviridae Proteins (metabolism)