Qualitative study of fecal alpha 1-proteinase inhibitor in normal subjects and patients with Crohn's disease.

Abstract

We applied sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting to analyze fecal alpha 1-proteinase inhibitor (alpha 1 PI) from healthy subjects and patients with Crohn's disease. A component with Mr 38,000 was characterized in normal fecal extracts as well as in six pathological samples. In these cases, the Crohn's disease activity index (CDAI), a clinical index of severity of the disease, was 170 (SEM 47). In contrast, alpha 1 PI of Mr 51,000 was detected in fecal extracts from eight patients with active Crohn's disease (CDAI = 287, SEM 39). We conclude that fecal alpha 1 PI can be considered a marker of intestinal disease activity.

• Authors: C Mizon, C Becuwe, M Balduyck, J F Colombel, A Cortot, J Mizon, P Degand
• Journal: Clinical chemistry (Clin Chem) Vol. 34 Issue 11 Pg. 2268-70 (Nov 1988) ISSN: 0009-9147 [Print] England
• PMID: 3263222 (Publication Type: Journal Article)

Chemical References

• Blood Proteins
• alpha 1-Antitrypsin

Topics

• Adolescent
• Adult
• Blood Proteins (analysis)
• Crohn Disease (metabolism)
• Electrophoresis, Polyacrylamide Gel
• Feces (analysis)
• Female
• Humans
• Immunoelectrophoresis, Two-Dimensional
• Male
• Middle Aged
• Molecular Weight
• alpha 1-Antitrypsin