Effect of a Single Application of CPP-ACPF Varnish on the Prevention of Erosive Tooth Wear: An AAS, AFM and SMH Study.

Abstract

PURPOSE:

The aim of this study was to investigate the in vitro effect of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride-containing varnish on prevention enamel erosive tooth wear.

MATERIALS AND METHODS:

A total of 28 enamel samples were prepared from human molars, divided into four groups: CPP-ACPF varnish, TCP-F varnish, NaF varnish, and deionised water. For the remineralisation process stimulated human pooled saliva was used. After treatment, all enamel samples were exposed to 10 ml of Coca Cola. Ca++ release was determined by atomic absorption spectroscopy (AAS). The surface topography was evaluated by atomic force microscopy (AFM). Surface microhardness of enamel was analysed and SMHR % (surface microhardness reduction ) was calculated. Data were analysed with repeated measures analysis of variance (ANOVA).

RESULTS:

Deionised water demonstrated a statistically significantly higher Ca+2 release compared to those of groups NaF > fTCP > CPP-ACPF, respectively (p <0.01). All groups measured for root-mean-square-roughness (Rrms) showed a statistically significantly difference of 6 × 6 μm2 and 12 × 12 μm2 enamel area (p <0.05) compared with a negative control group. CPP-ACPF varnish showed rougher surfaces than all remineralisation groups. SMHR % of enamels were as follows: CPP-ACPF < fTCP < NaF < deionised water (p <0.01).

CONCLUSION:

According to the findings of this study; CPP-ACP containing agents have a statistically statistically significant effect on preventing dental erosion. Among these, CPP-ACPF-containing remineralisation agents have the most effect on the remineralisation process.

• Authors: Berna Gokkaya, Nil Ozbek, Zeliha Guler, Suleyman Akman, A Sezai Sarac, Betul Kargul
• Journal: Oral health & preventive dentistry (Oral Health Prev Dent) Vol. 18 Issue 1 Pg. 311-318 (Apr 01 2020) ISSN: 1757-9996 [Electronic] Germany
• PMID: 32618454 (Publication Type: Journal Article)

Chemical References

• Caseins

Topics

• Humans
• Caseins
• Dental Enamel
• Microscopy, Atomic Force
• Paint
• Tooth Remineralization
• Tooth Wear