Abstract | BACKGROUND: Microorganisms produce siderophores in order to scavenge iron from the environment and this study focuses on the characterization of the two siderophores secreted by Bacillus megaterium. The general biological properties and pharmacokinetics following oral application of these compounds are reported. METHODS: RESULTS: Both compounds demonstrate strong antioxidant activity and were found to be relatively nontoxic to both human hepatocellular carcinoma (Huh7) and peripheral blood mononuclear cells. The siderophores possess a strong affinity for iron(III) and decrease the levels of the labile iron pool (LIP) in iron-loaded cells in a concentration-dependent manner. Schizokinen, was detected as both the free siderophore and the iron complex in the plasma and urine of rats after oral gavage. CONCLUSIONS: GENERAL SIGNIFICANCE: By virtue of the high affinity of schizokinen for tribasic metals, this siderophore does have considerable potential for the chelation of gallium(III) and the development of clinical diagnostic reagents.
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Authors | Hataichanok Chuljerm, Manu Deeudom, Suthat Fucharoen, Francesca Mazzacuva, Robert C Hider, Somdet Srichairatanakool, Agostino Cilibrizzi |
Journal | Biochimica et biophysica acta. General subjects
(Biochim Biophys Acta Gen Subj)
Vol. 1864
Issue 10
Pg. 129670
(10 2020)
ISSN: 1872-8006 [Electronic] Netherlands |
PMID | 32565293
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier B.V. All rights reserved. |
Chemical References |
- Antioxidants
- Hydroxamic Acids
- Iron Chelating Agents
- Siderophores
- schizokinen
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Topics |
- Animals
- Antioxidants
(chemistry, pharmacokinetics, pharmacology)
- Bacillus megaterium
(chemistry)
- Cell Line, Tumor
- Cells, Cultured
- Hydroxamic Acids
(chemistry, pharmacokinetics, pharmacology)
- Iron Chelating Agents
(chemistry, pharmacokinetics, pharmacology)
- Male
- Rats, Sprague-Dawley
- Siderophores
(chemistry, pharmacokinetics, pharmacology)
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