Dynamic alterations in serum IgG N-glycan profiles in the development of colitis-associated colon Cancer in mouse model.

Abstract

BACKGROUND:

Alternative glycosylation of serum IgG has been shown to be closely associated with colorectal cancer (CRC). Currently, a dynamic study which can not only minimize the influence of genetic background, environment and other interfering factors during cancer development, but also focus on investigating carcinogenic characteristics of IgG glycan is lacking.

METHODS:

Serum IgG N-glycans were characterized at four stages of CRC development by ultra-performance liquid chromatography in a typical colitis-related CRC mouse model induced by azoxymethane-dextran sodium sulfate. Furthermore, the expression of related glycosyltransferases in splenic B lymphocytes at the corresponding time was also assessed.

RESULTS:

The relative abundance of seven IgG glycans, which can be classified as monoantennary, core fucose, sialic acid, galactose and bisecting, was changed during tumor growth. The abundance of some glycans was altered during the first stage of cancer induction. Correspondingly, the expression of glycosyltransferases in splenic B lymphocytes and different tissues in cancer groups was also decreased compared to that in controls.

CONCLUSIONS:

This study represents the comprehensive analysis of IgG glycosylation in the dynamic process of colitis-associated CRC. To our knowledge, this is the first report that the expression of glycosyltransferases in mouse splenic B lymphocytes is consistent or inconsistent with the alterations of IgG N-glycans, and the variation tendency is tissue nonspecific.

GENERAL SIGNIFICANCE:

Providing a novel approach to identify the IgG glycans related to the development of CRC and laying a foundation for research on structure and function of glycans using mouse.

• Authors: Yong Gu, Jing Han, Xin Liu, Yiqing Pan, Xiaoyan Xu, Jichen Sha, Shifang Ren, Jianxin Gu
• Journal: Biochimica et biophysica acta. General subjects (Biochim Biophys Acta Gen Subj) Vol. 1864 Issue 10 Pg. 129668 (10 2020) ISSN: 1872-8006 [Electronic] Netherlands
• PMID: 32553689 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Immunoglobulin G
• Polysaccharides
• glycosylated IgG

Topics

• Animals
• Colitis (blood, complications, pathology)
• Colorectal Neoplasms (blood, etiology, pathology)
• Disease Models, Animal
• Female
• Glycomics
• Glycosylation
• Immunoglobulin G (analysis, blood)
• Mice
• Polysaccharides (analysis, blood)