LncRNA PLAC 2 Is Downregulated in Osteosarcoma and Regulates Cancer Cell Proliferation Through miR-93.

Abstract

INTRODUCTION:

PLAC 2 is a tumor-suppressive lncRNA in glioma, while its roles in other types of cancer remain unclear. This study was carried out to explore the potential involvement of PLAC 2 in osteosarcoma (OS).

METHODS:

Expression levels of PLAC 2 in OS and paired non-tumor tissues from OS patients were determined by RT-qPCR. A follow-up study was performed to analyze the prognostic value of PLAC 2 for OS. Interactions between PLAC 2 and miR-93 were assessed by cell transfection, followed by RT-qPCR. Cell proliferation assay was performed to analyze cell proliferation.

RESULTS:

Our results showed that PLAC 2 was downregulated in OS tissues, and the high expression levels of PLAC 2 were associated with favorable overall survival of OS patients. MiR-93 was upregulated in OS tissues and its expression was inversely correlated with the expression of PLAC 2. In OS cells, overexpression of PLAC 2 resulted in downregulated miR-93, while overexpression of miR-93 did not affect the expression of PLAC 2. Overexpression of PLAC 2 led to decreased proliferation rate of OS cells, while overexpression of miR-93 showed opposite roles and reduced the overexpressing effects of PLAC 2.

CONCLUSION:

PLAC 2 is downregulated in OS and regulates cancer cell proliferation through miR-93.

• Authors: Xiangran Sun, Ling Yu, Yubo Shi, Weichun Guo
• Journal: Cancer management and research (Cancer Manag Res) Vol. 12 Pg. 3623-3629 ( 2020) ISSN: 1179-1322 [Print] New Zealand
• PMID: 32547199 (Publication Type: Journal Article)
• Copyright: © 2020 Sun et al.