Systemic modulation of stress and immune parameters in patients treated for prostate adenocarcinoma by intensity-modulated radiation therapy or stereotactic ablative body radiotherapy.

Abstract

BACKGROUND:

In this exploratory study, the impact of local irradiation on systemic changes in stress and immune parameters was investigated in eight patients treated with intensity-modulated radiation therapy (IMRT) or stereotactic ablative body radiotherapy (SABR) for prostate adenocarcinoma to gain deeper insights into how radiotherapy (RT) modulates the immune system.

PATIENTS AND METHODS:

RT-qPCR, flow cytometry, metabolomics, and antibody arrays were used to monitor a panel of stress- and immune-related parameters before RT, after the first fraction (SABR) or the first week of treatment (IMRT), after the last fraction, and 3 weeks later in the blood of IMRT (N = 4) or SABR (N = 4) patients. Effect size analysis was used for comparison of results at different timepoints.

RESULTS:

Several parameters were found to be differentially modulated in IMRT and SABR patients: the expression of TGFB1, IL1B, and CCL3 genes; the expression of HLA-DR on circulating monocytes; the abundance and ratio of phosphatidylcholine and lysophosphatidylcholine metabolites in plasma. More immune modulators in plasma were modulated during IMRT than SABR, with only two common proteins, namely GDF-15 and Tim‑3.

CONCLUSION:

Locally delivered RT induces systemic modulation of the immune system in prostate adenocarcinoma patients. IMRT and SABR appear to specifically affect distinct immune components.

• Authors: B Frey, J Mika, K Jelonek, L Cruz-Garcia, C Roelants, I Testard, N Cherradi, K Lumniczky, S Polozov, A Napieralska, P Widlak, U S Gaipl, C Badie, J Polanska, S M Candéias
• Journal: Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al] (Strahlenther Onkol) Vol. 196 Issue 11 Pg. 1018-1033 (Nov 2020) ISSN: 1439-099X [Electronic] Germany
• PMID: 32519025 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Biomarkers
• Cytokines
• HLA Antigens
• Inflammation Mediators
• Lysophosphatidylcholines
• Neoplasm Proteins
• Phosphatidylcholines
• Proteome

Topics

• Adenocarcinoma (immunology, physiopathology, radiotherapy, surgery)
• Aged
• Aged, 80 and over
• Biomarkers
• Cytokines (blood)
• Gene Expression Regulation, Neoplastic (radiation effects)
• HLA Antigens (blood)
• Humans
• Immune System (radiation effects)
• Inflammation Mediators (blood)
• Lysophosphatidylcholines (blood)
• Male
• Metabolome (radiation effects)
• Middle Aged
• Monocytes (immunology)
• Neoplasm Proteins (blood)
• Phosphatidylcholines (blood)
• Prostatic Neoplasms (immunology, physiopathology, radiotherapy, surgery)
• Proteome (radiation effects)
• Radiosurgery (methods)
• Radiotherapy, Intensity-Modulated (methods)
• Stress, Physiological (radiation effects)