FLAER-negative CD15+ neutrophils can be used for the simplified screening of suspected PNH cases.

Abstract

BACKGROUND:

The flow cytometry analysis of GPI-linked proteins on red blood cells and leukocytes is crucial for paroxysmal nocturnal hemoglobinuria (PNH) diagnostics. However, the commonly used multicolor panels cannot be implemented in low-resourced hematology laboratories. In order to develop a simple prediagnostic test for PNH screening, we analyzed the diagnostic accuracy of the two-color (FLAER/CD15) detection of GPI-deficient neutrophils.

METHODS:

We reanalyzed multicolor data set of 1594 peripheral blood samples of patients screened for PNH applying only two markers (FLAER/CD15). The quantitative positivity/negativity was reported. Then, these results were compared in a blinded manner with previously obtained multicolor data from the same samples.

RESULTS:

Among the 1594 samples included in the study, 507 samples were PNH-positive by the multicolor assay. The two-color method revealed 510 PNH-positive samples. The detailed examination of this discrepancy revealed 12 false-positives and 9 false-negatives. Therefore, FLAER/CD15 screening method displayed 98.90% of the diagnostic specificity and 98.22% of the sensitivity.

CONCLUSION:

This simple two-color evaluation of FLAER-negative neutrophils is a highly effective screening test for PNH. Although this approach is not intended to replace the multicolor diagnostic procedure, it could minimize the number of patients requiring a conventional multicolor flow cytometric assay.

• Authors: Alexandra Semchenkova, Svetlana Kashpor, Tatiana Verzhbitskaya, Vyacheslav Borisov, Olga Illarionova, Larisa Fechina, Alexey Maschan, Galina Novichkova, Alexander Popov
• Journal: International journal of laboratory hematology (Int J Lab Hematol) Vol. 42 Issue 5 Pg. 589-593 (Oct 2020) ISSN: 1751-553X [Electronic] England
• PMID: 32449605 (Publication Type: Journal Article)
• Copyright: © 2020 John Wiley & Sons Ltd.

Chemical References

• Biomarkers
• GPI-Linked Proteins
• Lewis X Antigen

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Biomarkers
• Child
• Child, Preschool
• Female
• Flow Cytometry
• GPI-Linked Proteins (metabolism)
• Hemoglobinuria, Paroxysmal (blood, diagnosis, metabolism)
• Humans
• Lewis X Antigen (metabolism)
• Male
• Middle Aged
• Neutrophils (metabolism)
• Young Adult