Development of a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) for measuring venom antigens after an experimental snake bite.

We describe a new ELISA which allows the measurement of the concentration of venom antigens in whole blood. The assay can be performed in less than 20 min and requires a 200 microliters sample of blood. It allows the accurate evaluation of concentrations of Vipera ammodytes venom in quantities smaller than 1 ng/ml of blood. Using this ELISA, we were able to follow in rabbits the kinetics of experimental envenomation with non-lethal doses of venom. This ELISA was also used to measure post mortem the level of venom antigens in various tissues such as liver, kidney, muscles and abdominal serosity of a rabbit. The method, which might be adapted to measure envenomation by other snake species, seems to be sufficiently rapid and sensitive to allow routine evaluation of the gravity of a snake bite in humans and to estimate the efficacy of immunotherapy.
AuthorsH Labrousse, A K Nishikawa, C Bon, S Avrameas
JournalToxicon : official journal of the International Society on Toxinology (Toxicon) Vol. 26 Issue 12 Pg. 1157-67 ( 1988) ISSN: 0041-0101 [Print] ENGLAND
PMID3238700 (Publication Type: Journal Article)
Chemical References
  • Antigens
  • Snake Venoms
  • Animals
  • Antigens (analysis)
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Immunization, Passive
  • Kinetics
  • Male
  • Mice
  • Rabbits
  • Snake Bites (diagnosis, therapy)
  • Snake Venoms (analysis, immunology)

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