Abstract |
The before introduced solid phase ELISA was employed for the RF determination in the sera of RA patients and controls. The threshold values for positive results (calculated as the 95% distribution percentile of healthy donors) were 8, 3, and 3 U/ml for IgA, IgG, and IgM-RF, respectively. The results confirm the validity of the assay with clear negative results in several negative control groups (healthy donors, patients of the oto-, rhino-, laryngeal ambulance, diabetes mellitus, degenerative arthropathies; n = 111, median IgA, IgG and IgM-RF values of less than or equal to 2, less than or equal to 2 and less than or equal to 1 U/ml, respectively; 25-75% distribution percentiles within the median value) and positive results in the positive control group (seropositive RA; n = 20, median IgA, IgG and IgM-RF values of 324, 479 and 170 U/ml, respectively). 16/24 patients with so-called seronegative RA (negative Latex Fixation Test or Waaler Rose Test) had positive results in the ELISA, two of them had rheumatoid nodules clinically. The IgG-RF activity in the ELISA appears to be a good parameter for the course control of RA under gold therapy. 10 RA patients with clinical improvement of disease (declining ESR, CRP, joint index) after six months of gold therapy (= 0.6 g total gold amount) had a decline of total RF activity of 70% in median, whereas 10 patients with no clear effect on disease activity had only a decline of 20% in median.
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Authors | G Lemm, S Ruschen, H Warnatz |
Journal | Scandinavian journal of rheumatology. Supplement
(Scand J Rheumatol Suppl)
Vol. 75
Pg. 256-60
( 1988)
ISSN: 0301-3847 [Print] Norway |
PMID | 3238359
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunoglobulin A
- Immunoglobulin G
- Immunoglobulin M
- Gold
- C-Reactive Protein
- Rheumatoid Factor
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Topics |
- Arthritis, Rheumatoid
(blood, drug therapy)
- Blood Sedimentation
- C-Reactive Protein
(analysis)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Gold
(therapeutic use)
- Humans
- Immunoglobulin A
(analysis, immunology)
- Immunoglobulin G
(analysis, immunology)
- Immunoglobulin M
(analysis, immunology)
- Rheumatoid Factor
(analysis)
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