In Vivo Targets of Pasteurella Multocida Toxin.

Abstract

Many Pasteurella multocida strains are carried as commensals, while some cause disease in animals and humans. Some type D strains cause atrophic rhinitis in pigs, where the causative agent is known to be the Pasteurella multocida toxin (PMT). PMT activates three families of G-proteins-Gq/11, G12/13, and Gi/o-leading to cellular mitogenesis and other sequelae. The effects of PMT on whole animals in vivo have been investigated previously, but only at the level of organ-specific pathogenesis. We report here the first study to screen all the organs targeted by the toxin by using the QE antibody that recognizes only PMT-modified G-proteins. Under our experimental conditions, short-term treatment of PMT is shown to have multiple in vivo targets, demonstrating G-alpha protein modification, stimulation of proliferation markers and expression of active β-catenin in a tissue- and cell-specific manner. This highlights the usefulness of PMT as an important tool for dissecting the specific roles of different G-alpha proteins in vivo.

• Authors: Arshiya Banu, Alistair J Lax, Agamemnon E Grigoriadis
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 21 Issue 8 (Apr 15 2020) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 32326543 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• Bacterial Toxins
• Pasteurella multocida toxin
• Recombinant Proteins
• beta Catenin
• GTP-Binding Protein alpha Subunits, Gq-G11

Topics

• Animals
• Bacterial Proteins (genetics, toxicity)
• Bacterial Toxins (genetics, toxicity)
• Cell Proliferation (drug effects)
• Endometrium (drug effects, metabolism)
• Female
• GTP-Binding Protein alpha Subunits, Gq-G11 (metabolism)
• Immunohistochemistry
• Mice
• Pasteurella multocida (metabolism)
• Recombinant Proteins (genetics, metabolism)
• Signal Transduction (drug effects)
• Spleen (drug effects, metabolism)
• Thymus Gland (drug effects, metabolism)
• Uterus (drug effects, metabolism)
• beta Catenin (metabolism)